Accession Number : AD0002144


Title :   THE LABORATORY PRODUCTION OF GONYAULAX CATANELLA POISON


Corporate Author : ARMY BIOLOGICAL LABS FREDERICK MD


Personal Author(s) : GRAF, LLOYD H ; THACHER, MABEL J ; BREWER, CARL R


Full Text : https://apps.dtic.mil/dtic/tr/fulltext/u2/002144.pdf


Report Date : 06 Nov 1952


Pagination or Media Count : 50


Abstract : Methods were developed for estimating Gonyaulax catanella growt h by direct microscopic count or by the analytical determination of cellular carbonhydrate. Use of a large inoculum, illumination with fluorescent light, and continuous incubation at 17 deg C for 7-14 days gave maximal yields of 50,000- 80,000 cells/ml in sea water and in artificial sea-water media. The addition of culture filtrates, extracts of contaminant bacteria, or organic nutrients to sea-water media did not alter growth. Addition of polymyxin B, penicillin, streptothricin, or streptomycin improved the growth. Contact with rubber products or galvanized sheet metal completely inhibited growth. Stainless steel and polyethylene inhibition were overcome with ethylene-diaminetetraacetate (EDTA); neither Al nor saran caused inhibition. Growth in the sea salt medium was improved by the addition of 0.01% EDTA and 5-15% of sea water. The contaminants in the stock G, catanella culture were not eliminated by treatment with antibiotics, washing processes, or UV irradiation. The best poison yield, 1.3 mouse units/ml, was obtained in 3.5 l of sea-water culture containing 17 Gamma of polymyxin B/ml after 7 days of incubation in a rotating fermenter with continuous fluorescent illumination.


Descriptors :   *MUSSEL POISONS , FLUORESCENCE , COUNTING METHODS , NUTRIENTS , YIELD , CONTAMINANTS , INCUBATION , FERMENTATION , INHIBITION , SEA WATER , CULTURES(BIOLOGY) , FILTRATION


Subject Categories : Toxicology


Distribution Statement : APPROVED FOR PUBLIC RELEASE